At initial uses, hold the tab of the stopper ring and tear it off in the direction of the arrow to open the freshness seal. Discard the stopper ring after removal. Apply after balancing lotion. Can be layered with any additional serums, followed by moisturizer. Press twice to dispense serum onto fingertips and smooth gently over areas of concern, paying special attention to wrinkle-prone areas including the skin around the eyes and mouth. Use of a UV protection product is recommended after the application of the serum each morning. If you have sensitive skin, apply serum 2-3 evenings per week for the first 2 weeks and gradually increase application to nightly for the next two weeks, then twice a day. Avoid use if you skin is sensitive or if you have previously had adverse reactions to products containing retinol. Avoid simultaneous use of other products containing retinol. Carefully monitor skin for sensitivity before the application of any other formulations.