11月29日 Pierre-Antoine Defossez:Genetic and structural investigations of DNA methylation maintenance mechanisms


报告题目:Genetic and structural investigations of DNA methylation maintenance mechanisms

报告人:Pierre-Antoine Defossez 巴黎第七大学

主持人:翁杰敏 教授

报告时间:2019年11月29日10:00(周五上午)

报告地点:腾讯时时彩官网开奖534小会议室


报告人简介:

Current position

French public organisation

RNSR code

Organisation

Laboratory

Unit code

Postcode

Town

200918506F

Professor,CNRS/Univ Paris Diderotor

  

Epigenetics and cell fate

7216

75013

Paris

Function

Group leader, DR2 CNRS, since 2009.

Other activities

Executive board, supervision of student, teaching, memberships in panels or individual scientific reviewing activities

Associate professor, Ecole Polytechnique.

Teaching activity at ENS Paris, ENS Cachan, and ENS Lyon.

CNRS study section 21, member 2012-2015.
ARC study section 3, member 2012-2016.

Institut National du Cancer, PLBio evaluation committee, member in 2011, president in 2012 and 2017.

Executive board, Labex “Who am I?”

Reviewer for multiple national and international research agencies: HCERES, ANR, ARC, Ligue, INCa (France); Cancer Research UK, MRC, BBSRC (UK); NWO (Netherlands); FWF (Austria); CIHR (Canada).

Ad hoc reviewer for: EMBO Journal, Genome Research, Elife, Nucleic Acids Research, Nature Communications, Cell Reports, Molecular Cell, Nature…

Previous positions

Start date

End date

Town

Organisation

Function

04/2003

12/2008

Paris, France

Institut Curie, Department of Epigenetics

(Head: Geneviève Almouzni)

Junior group leader

07/2000

04/2003

Lyon, France

ENS Lyon, lab of Eric Gilson

Research Scientist

01/1997

06/2000

Cambridge, USA

MIT, lab of Lenny Guarente

Postdoc

Education

Ecole Normale Supérieure, Paris, 1990-1993

PhD, Lille, 1996

HDR 2003











报告内容:

DNA methylation is an essential epigenetic mark in mammals, and it needs to be maintained at each round of DNA replication. Two key actors in this epigenetic maintenance process are the DNA methyltransferase DNMT1, and an E3 ubiquitin ligase acting upstream of DNMT1, called UHRF1. How UHRF1 itself is recruited to replicating chromatin is incompletely understood. One of the mechanisms that was put forward is an interaction between the Tandem Tudor Domain (TTD) of UHRF1 and histone H3K9me2/3. Recently, we identified a new and unexpected mechanism that directly couples DNA replication and UHRF1 recruitment: a methylated histone-like region of DNA Ligase 1 (LIG1K126me2/me3) binds the UHRF1 TTD with nanomolar affinity, permitting UHRF1 recruitment to chromatin. We will report our recently obtained structure of the LIG1/UHRF1 complex, which sheds light on its regulation. We will also report on our unbiased CRISPR screen aimed at identifying new factors involved in DNA methylation maintenance.


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